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KMID : 0378019630060060065
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New Medical Journal
1963 Volume.6 No. 6 p.65 ~ p.71
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Preparation of Standard Thromboplastin Utilizng Human Brain
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Abstract
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1. The author has tried to prepare a standard thromboplastin for the determination of prothrombin time i utilizing relatively fresh child human brain obtained at the time of autopsy, through acetone i treatment.
2. A 13.5 second thromboplastin extract, slightly slower than the 12 second commercially available Difco product, was successfully prepared.
3. No influence on prothrombin time is noted when the thromboplastin solution is extracted from the powdered thromboplastin at the temperature, between 45C and 55 C. However, definite prolongation is observed when the extraction is made at above 60C.
4. It appears that the optimal concentrations of powdered thromboplastin for the preparation of thromboplastin solution lie between 300 mg and 500 mg.
5. An attempt was made to prepare a slower acting thromboplastin sensitive at the level of therapeutic range, using rabbit brain and lung extract in equal parts, with uncertain success.
6. It is felt that the prospect of preparing a satisfactory fast acting standard thromboplastin is good, judging from the knowledge acquired through this experimentation. At this time, however, we are unable to draw any specific conclusion concerning the preparation of a sensitive slower acting thromboplastin from the rabbit.
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